Akta purifier 10 manual

 · AKTA PURIFIER MANUAL PDF. About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse. Tubing Kit Changes - ÄKTApurifier 10 GENERAL FLOW (at delivery) mm i.d Blue PEEK Outlet Valve V4 (7ul) FR (Mpa, 5ul) pH Flow cell (ul) Accumulator (40ul) UV Flow cell Conductivity Flow cell (14ul) 1 0 0 ul G6 G7 G8 G9 Injection valve V1 G5 Tubing Kit No Frac Frac Frac AKTApurifier 10, tubing kit This method is more accurate than the one described above, but demands one more system. 1) Check that the pump on your chosen ÄKTA system is delivering the correct flow at 1 ml/min. If the measured flow rate differs the retention volumes need to be corrected. 2) Mount a small loop, e.g. µl, and prepare a solution of % acetone to use as.

The GE AKTA Purifier 10 FPLC System is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. A KTA Purifier 10 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of to 10 ml/min at pressures of 0 to 25 MPa. Start a run manually at 0% B. Make sure the switch valve is set correctly to the inlet A1. ÄKTApurifier User guide Edition AC 93 5 Optional functions BufferPrep 2 Check the pH reading when stable. 3 Change to %B by setting the Gradient instruction in Manual:Pump to %B for Target and 0 for Length. Clean the flow cell according to instructions in the Monitor pH/C User Manual. Leaking tubing connections. Tighten the connectors. If necessary replace the connectors. Please refer to the order information in the System Manual. Poor mixing of buffers. Check the mixer function by placing a stirrer bar on top of the mixer housing.

Note: Always position the optical unit with the filter wheel cover facing upwards. Run the pump manually at 10 ml/min with water. Note the. All users must read this entire manual to fully understand the safe use of ÄKTA™ monitor UPC Workstation. WARNING! The WARNING! sign highlights instructions. 16 de mai. de All users must read this entiremanual to fully understand the safe www.doorway.ru AKTA PurifUPC, and UPC specified to your Monday June.